A planned cultural and education centre at the Olympic Park is to receive £141m funding, the Treasury has said.
The spending has been announced in the National Infrastructure Planpublished ahead of the chancellor's Autumn Statement.
Also announced were £150m for housing revamps in four London boroughs and £55m for a rail extension in east London.
The money is part of a package worth almost £350m to London.
The Victoria and Albert Museum and Sadler's Wells are planning to be a part of Olympicopolis, while University College London will move to a site south of the Orbit sculpture.
The spending announcement included plans to extend the London Overground, creating a new rail link to London's largest regeneration scheme at Barking Riverside.
The Mayor of London Boris Johnson said the Olympicopolis project would generate millions of pounds for east London's economy and support thousands of jobs.
He said: "This is an important milestone in our plans to realise a legacy from London's 2012 Games that reaps economic and social dividends to last for generations to come."
Labour is yet to comment. BBC NEWS
